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Cell Culture    |    Cell Staining    |    Molecular Biology    |    Protein Biochemistry    |    Vector Maps    |    Miscellaneous
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Cell Culture

293 Gene Targeting (A648) and NHEJ (1040) Cell Transfections
Cell Culture Plate Surface Areas
FACS Machine Setup
Freezing Down Mammalian Cells for Long-Term Storage
Invitrogen Antibiotic Selection Guide (Useful Concentrations for Specific Drugs / Cell Types)
Soft-Agar Growth Assay
Splitting Cells
Thawing Mammalian Cells

 

Cell Staining

Crystal Violet Staining of Cell Colonies
Immunofluorescence using 3% PFA and 0.1% Saponin
Immunofluorescence using 3% PFA and 0.2% Triton X-100
Immunofluorescence using Methanol and 0.1% Saponin
Metaphase Chromosome Spreads
Sister Chromatid Exchange (SCE) Assay
Telomere Chromosome Orientation FISH (CO-FISH)
Telomere FISH

 

Molecular Biology

Applied Biosystems QPCR Manual
Innoculating Bacterial Liquid Cultures
Ligation / Transformation / Mini-Prep / Sequencing
Ligation-Mediated PCR (LM-PCR)
Making Competent Cells
Phusion PCR
Real Time Quantitative PCR Manual (Current Protocols)
Southern Blotting
Superscript III Reverse Transcription
Transforming Yeast
XL10-Gold Bacterial Transformation

 

Protein Biochemistry

Abcam Immunoprecipitation (IP) Protocol
Cell Fractionation (Cytoplasm / Nucleus / Nucleolus) from the Lamond Lab
Co-Immunoprecipitation (Co-IP) from Transfected HeLa Cells
Covalent Coupling of Antibodies to Protein A Beads
Denaturing Nickel Bead Pulldown of His-tagged Proteins from Mammalian Cells
GST-Protein Purification from Bacteria
In vitro Pulldown of Recombinant GST-Protein with in vitro Translated Protein
In vitro Pulldown of Recombinant GST-Protein with Recombinant Untagged Protein
In vitro Pulldown of Recombinant His-Protein with in vitro Translated Protein
In vitro Translation with Non-Radioactive Methionine
In vitro Translation with Radioactive 35S-Methionine
Large Scale Immunoprecipitation
Making Protein Lysates from Cells
Measuring Protein Concentration in Cell Lysates with BCA Kit
Measuring Protein Concentration in Cell Lysates with BCA Kit Excel Calculation
Pouring SDS-PAGE Gels
Protein Transfer Guide
Running Resource Q Column
SDS-PAGE Gel Recipes
Semi-Dry Transfer and Western Blotting
Tandem Affinity Purification (TAP)
Ubiquitin E2 Charging Assay

 

Vector Maps

pCS2-eGFP
pCS2-HA
pCS2-mCherry
pCS2-Myc
pCS2-Untagged
pGEX6p1-GST
pIRESpuro-FLAG
pIRESpuro-TAP

 

Miscellaneous

Buffer and Reagent Recipes
Statistical Calculations with Excel

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